Composite

Part:BBa_K519012:Design

Designed by: Kotone Miyake   Group: iGEM11_Tokyo-NoKoGen   (2011-09-30)

Promoter and RBS with PduP1~18 tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N-terminal of PduP is expressed under a promoter and RBS


Source

Citrobacter freundii

Citrobacter freundii is not infectious.

References

"Short N-terminal sequence package proteins into bacterial microcompartments", Chenguang Fan et al., PNAS, April 20 1010, vol.107 no.16 7509-7514